This chapel is dedicated to the Apostle of the Gentiles who brought the Christian faith to the Maltese in AD 60. In this altarpiece, he is shown praying to the Blessed Virgin Mary under the title of the Immaculate Conception just after his shipwreck on the island, an iconography which is quite unusual in local art. The painting is by Stefano Erardi and was commissioned in the late seventeenth century.

On the right-hand side of the altar stands a marble and bronze monument of Archpriest Alfons Maria Hili, who led the parish in the early twentieth century. As an outstanding benefactor of the basilica, he was responsible for the building of the side aisles in the 1930s.

This chapel is intended as a refuge for prayer, with the representation of the Crucified Christ – Pietru Pawl Azzopardi’s masterpiece – acting as a focus of contemplation. The miraculous wooden statue, made in 1848, is taken out with a set of statues representing various stations of the Way of the Cross, in the oldest Good Friday procession on the island.